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Alpha & Beta Studies: RTFaSt™ Tissue Processing Innovation ValidatedProven. Tested. Clinically Trusted.

The development of RTFaSt™ (Rapid Tissue Fixation and Staining) has been guided by rigorous alpha and beta testing to ensure one goal: to deliver faster, higher-quality histological results without compromising diagnostic accuracy.

From Concept to Clinical Proof:
The Journey

RTFaSt™ began as a bold idea to revolutionize tissue processing using a patented reagent system compatible with paraffin embedding. Through a two-phase testing approach—Alpha (proof-of-concept) and Beta (clinical optimization)—this vision has now become a laboratory-ready solution.

Alpha Studies (animal):
Proof of Performance

Objective

Validate effectiveness of RTFaSt™ fixatives for paraffin-embedded tissues

Process

Compared to traditional formalin fixation and processing

Findings

  • Tissue sections showed clear cellular morphology

  • No need for extended pre-fixation

  • Set the stage for faster paraffin workflow protocols

Result: Strong feasibility confirmed — RTFaSt™ reagents ready for expanded clinical testing

Beta Studies (human):

Optimizing for Real-World Labs

1 / Location

Ukraine

2 / Evaluator

Pathologist-supervised team with validated SOPs

3 / Goal

Optimize RTFaSt™ protocol for different tissue sizes using microwave vacuum-assisted closed processors

Key Beta Findings

Protocol
Processing time
Quality
Standard (Control)
~22 hours
Optimal
RTFaSt1A/B (Xylene-based)
1.7–3.9 hours
Optimal – matched control
RTFaSt2A/B (Toluene-based)
1.7–3.9 hours
Sub-optimal – tissue artifact noted
“RTFaSt 1A/B slides were of optimal quality, identical to control slides produced by traditional processing"

Data Test Final Evaluation Report, Medi-Sci Consultants

Equivalent H&E quality vs. 22-hour formalin workflows

Massive time savings (up to 80% faster)

Cost savings from reduced reagent use and standard time

Eco-friendly lower chemical waste and lower energy

What We Learned

RTFaSt1A/B is the recommended clinical protocol: moderate heat + rapid xylene
yields best results

RTFaSt2A/B (toluene) introduced processing artifacts like blue haze, tissue
shredding, and water retention

Microwave vacuum-assisted tissue processing is clinically viable when
combined with RTFaSt reagents

Ready for Real-World Use

With support from histotechnologists, MD evaluators, and diagnostic pathologists, RTFaSt™ is not just an innovation—it's a validated clinical tool ready to modernize pathology labs across the globe.

Let’s Bring Innovation to Your Lab

Discover the science behind the speed. Explore the RTFaSt™ advantage.

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