Alpha & Beta Studies: RTFaSt™ Tissue Processing Innovation ValidatedProven. Tested. Clinically Trusted.
The development of RTFaSt™ (Rapid Tissue Fixation and Staining) has been guided by rigorous alpha and beta testing to ensure one goal: to deliver faster, higher-quality histological results without compromising diagnostic accuracy.


From Concept to Clinical Proof:
The Journey
RTFaSt™ began as a bold idea to revolutionize tissue processing using a patented reagent system compatible with paraffin embedding. Through a two-phase testing approach—Alpha (proof-of-concept) and Beta (clinical optimization)—this vision has now become a laboratory-ready solution.
Alpha Studies (animal):
Proof of Performance

Objective
Validate effectiveness of RTFaSt™ fixatives for paraffin-embedded tissues

Process
Compared to traditional formalin fixation and processing

Findings
-
Tissue sections showed clear cellular morphology
-
No need for extended pre-fixation
-
Set the stage for faster paraffin workflow protocols
Result: Strong feasibility confirmed — RTFaSt™ reagents ready for expanded clinical testing
Beta Studies (human):
Optimizing for Real-World Labs
1 / Location
Ukraine
2 / Evaluator
Pathologist-supervised team with validated SOPs
3 / Goal
Optimize RTFaSt™ protocol for different tissue sizes using microwave vacuum-assisted closed processors
Key Beta Findings
Protocol | Processing time | Quality |
---|---|---|
Standard (Control) | ~22 hours | Optimal |
RTFaSt1A/B (Xylene-based) | 1.7–3.9 hours | Optimal – matched control |
RTFaSt2A/B (Toluene-based) | 1.7–3.9 hours | Sub-optimal – tissue artifact noted |
Equivalent H&E quality vs. 22-hour formalin workflows
Massive time savings (up to 80% faster)
Cost savings from reduced reagent use and standard time
Eco-friendly lower chemical waste and lower energy
What We Learned
RTFaSt1A/B is the recommended clinical protocol: moderate heat + rapid xylene
yields best results
RTFaSt2A/B (toluene) introduced processing artifacts like blue haze, tissue
shredding, and water retention
Microwave vacuum-assisted tissue processing is clinically viable when
combined with RTFaSt reagents

Ready for Real-World Use
With support from histotechnologists, MD evaluators, and diagnostic pathologists, RTFaSt™ is not just an innovation—it's a validated clinical tool ready to modernize pathology labs across the globe.